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Quantitative Proteomics Claire E. Eyers, ISBN-13: 978-1849738088

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Quantitative Proteomics: RSC (New Developments in Mass Spectrometry) by Claire E. Eyers, ISBN-13: 978-1849738088
[PDF eBook eTextbook]

Series: New Developments in Mass Spectrometry (Book 1)
390 pages
Publisher: Royal Society of Chemistry; 1 edition (February 11, 2014)
Language: English
ISBN-10: 9781849738088
ISBN-13: 978-1849738088

As a component of post-genome science, the field of proteomics has assumed great prominence in recent years. Whereas quantitative analyses focussed initially on relative quantification, a greater emphasis is now placed on absolute quantification and consideration of proteome dynamics. Coverage of the topic of quantitative proteomics requires consideration both of the analytical fundamentals of quantitative mass spectrometry and the specific demands of the problem being addressed.

Quantitative Proteomics aims to outline the state of the art in mass spectrometry-based quantitative proteomics, describing recent advances and current limitations in the instrumentation used, together with the various methods employed for generating high quality data. Details on both strategies describing how stable isotope labelling can be applied and methods for performing quantitative analysis of proteins in a label-free manner are given. The utility of these strategies to understanding cellular protein dynamics are then exemplified with chapters looking at spatial proteomics, dynamics of protein function as determined by quantifying changes in protein post-translational modification and protein turnover. Finally, a key application of these techniques to biomarker discovery and validation is presented, together with the rapidly developing area of quantitative analysis of protein-based foodstuffs.

This exemplary book is essential reading for analytical and biological mass spectrometrists working in proteomics research, as well as those undertaking either fundamental or clinical-based investigations with an interest in understanding protein dynamics and/or biomarker assessment.


“Quantitative Proteomics should not be regarded only as an updated reference book of cutting-edge bottom-up strategies for quantitative proteomics. Its reading makes us reflect on the enormous experimental progress made possible by the recent developments magnificently summarized in the 15 chapters in this new volume of the series New Developments in Mass Spectrometry.” (Juan J. Calvete Journal of Proteomics)

“the editors have assembled authors with the necessary knowledge combined with an ability to explain current practices whilst simultaneously stating the limitations. Most authors also propose new ideas and possible extensions that may well apply in the future.”
(K. Jones Chromatographia (2015) 78:141–142)

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